Review



biotinylated anti mouse slpi ab  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    R&D Systems biotinylated anti mouse slpi ab
    FIGURE 1. Expression of <t>SLPI</t> during mycobacterium infection. A, Wild-type mice were intratracheally infected with BCG (4 105 CFU). At the indicated periods, total RNA was extracted from the lungs. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. B and C, At 2 days after intratracheal infection with BCG, lung tissue sections were stained with an anti-SLPI Ab (red) and 4,6-diamidino-2-phenylindole (blue) and visualized by fluorescence microscopy. D, BALF was collected at the indicated periods after BCG infection. Mouse SLPI protein expression was analyzed by Western blotting with an anti-SLPI Ab. Data obtained from two independent mice (0, 1, and 2 days) are indicated. E, AEC were incubated with the same number of BCG for the indicated periods. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. F, AEC were incubated with the same number of BCG. Culture supernatants were collected before () and after 24 h of infection () and subjected to Western blot analysis using an anti-SLPI Ab. Data obtained from two independent cell clones are shown. G, Alveolar macrophages were collected from uninfected wild-type mice, cultured with or without BCG for 48 h, and then analyzed for their SLPI mRNA expression by quantitative real-time RT-PCR. The results are presented as the mean SD.
    Biotinylated Anti Mouse Slpi Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+mouse+slpi+ab/Mouse+SLPI+Biotinylated+Antibody/pm18322212-61-12-24
    Average 94 stars, based on 6 article reviews
    biotinylated anti mouse slpi ab - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor."

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.180.6.4032

    FIGURE 1. Expression of SLPI during mycobacterium infection. A, Wild-type mice were intratracheally infected with BCG (4 105 CFU). At the indicated periods, total RNA was extracted from the lungs. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. B and C, At 2 days after intratracheal infection with BCG, lung tissue sections were stained with an anti-SLPI Ab (red) and 4,6-diamidino-2-phenylindole (blue) and visualized by fluorescence microscopy. D, BALF was collected at the indicated periods after BCG infection. Mouse SLPI protein expression was analyzed by Western blotting with an anti-SLPI Ab. Data obtained from two independent mice (0, 1, and 2 days) are indicated. E, AEC were incubated with the same number of BCG for the indicated periods. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. F, AEC were incubated with the same number of BCG. Culture supernatants were collected before () and after 24 h of infection () and subjected to Western blot analysis using an anti-SLPI Ab. Data obtained from two independent cell clones are shown. G, Alveolar macrophages were collected from uninfected wild-type mice, cultured with or without BCG for 48 h, and then analyzed for their SLPI mRNA expression by quantitative real-time RT-PCR. The results are presented as the mean SD.
    Figure Legend Snippet: FIGURE 1. Expression of SLPI during mycobacterium infection. A, Wild-type mice were intratracheally infected with BCG (4 105 CFU). At the indicated periods, total RNA was extracted from the lungs. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. B and C, At 2 days after intratracheal infection with BCG, lung tissue sections were stained with an anti-SLPI Ab (red) and 4,6-diamidino-2-phenylindole (blue) and visualized by fluorescence microscopy. D, BALF was collected at the indicated periods after BCG infection. Mouse SLPI protein expression was analyzed by Western blotting with an anti-SLPI Ab. Data obtained from two independent mice (0, 1, and 2 days) are indicated. E, AEC were incubated with the same number of BCG for the indicated periods. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. F, AEC were incubated with the same number of BCG. Culture supernatants were collected before () and after 24 h of infection () and subjected to Western blot analysis using an anti-SLPI Ab. Data obtained from two independent cell clones are shown. G, Alveolar macrophages were collected from uninfected wild-type mice, cultured with or without BCG for 48 h, and then analyzed for their SLPI mRNA expression by quantitative real-time RT-PCR. The results are presented as the mean SD.

    Techniques Used: Expressing, Infection, Quantitative RT-PCR, Staining, Microscopy, Western Blot, Incubation, Clone Assay, Cell Culture

    FIGURE 3. SLPI associates with BCG. SLPI and BSA were labeled with FLUOS (Roche). Labeled proteins were incubated with BCG for 30 min, and analyzed by fluorescence microscopy.
    Figure Legend Snippet: FIGURE 3. SLPI associates with BCG. SLPI and BSA were labeled with FLUOS (Roche). Labeled proteins were incubated with BCG for 30 min, and analyzed by fluorescence microscopy.

    Techniques Used: Labeling, Incubation, Microscopy

    FIGURE 4. SLPI disrupts the BCG cell membrane. A, BCG was incubated with or without SLPI for the indicated periods and observed with scanning electron microscopy. B, The indicated concentrations of SLPI were added to a BCG suspension containing NPN, and the NPN fluorescence was moni- tored for the indicated periods. Repre- sentative data of three independent ex- periments are shown.
    Figure Legend Snippet: FIGURE 4. SLPI disrupts the BCG cell membrane. A, BCG was incubated with or without SLPI for the indicated periods and observed with scanning electron microscopy. B, The indicated concentrations of SLPI were added to a BCG suspension containing NPN, and the NPN fluorescence was moni- tored for the indicated periods. Repre- sentative data of three independent ex- periments are shown.

    Techniques Used: Membrane, Incubation, Electron Microscopy, Suspension

    FIGURE 7. Slpi/ mice are highly susceptible to M. tuberculosis infection. A, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/ mice and their survival was monitored. B, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/
    Figure Legend Snippet: FIGURE 7. Slpi/ mice are highly susceptible to M. tuberculosis infection. A, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/ mice and their survival was monitored. B, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/

    Techniques Used: Infection

    Related Articles

    Incubation:

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.
    Article Snippet: Cryostat sections (5- m thick) were fixed with cold acetone for 10 min, dried, rehydrated with PBS, and blocked with PBS containing 20 mM HEPES, 10% FBS, and 1 g of Fc-blocking mAb (2.4G2; BD Pharmingen). .. Next, the sections were sequentially incubated with a biotinylated anti-mouse SLPI Ab (R&D Systems) and Alexa Fluor 594-conjugated streptavidin (Molecular Probes). .. The nuclei were stained with 4 ,6-diamidino-2-phenylindole (Molecular Probes).

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.
    Article Snippet: The separated proteins were transferred to a 0.45- m pore polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems). .. The bound Abs were detected by the Super Signal reagent (Pierce).

    Blocking Assay:

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.
    Article Snippet: The separated proteins were transferred to a 0.45- m pore polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems). .. The bound Abs were detected by the Super Signal reagent (Pierce).

    Membrane:

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.
    Article Snippet: The separated proteins were transferred to a 0.45- m pore polyvinylidene fluoride membrane (Millipore). .. After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems). .. The bound Abs were detected by the Super Signal reagent (Pierce).



    Similar Products

    biotinylated anti mouse slpi ab  (R&D Systems)
    94
    R&D Systems biotinylated anti mouse slpi ab
    FIGURE 1. Expression of <t>SLPI</t> during mycobacterium infection. A, Wild-type mice were intratracheally infected with BCG (4 105 CFU). At the indicated periods, total RNA was extracted from the lungs. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. B and C, At 2 days after intratracheal infection with BCG, lung tissue sections were stained with an anti-SLPI Ab (red) and 4,6-diamidino-2-phenylindole (blue) and visualized by fluorescence microscopy. D, BALF was collected at the indicated periods after BCG infection. Mouse SLPI protein expression was analyzed by Western blotting with an anti-SLPI Ab. Data obtained from two independent mice (0, 1, and 2 days) are indicated. E, AEC were incubated with the same number of BCG for the indicated periods. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. F, AEC were incubated with the same number of BCG. Culture supernatants were collected before () and after 24 h of infection () and subjected to Western blot analysis using an anti-SLPI Ab. Data obtained from two independent cell clones are shown. G, Alveolar macrophages were collected from uninfected wild-type mice, cultured with or without BCG for 48 h, and then analyzed for their SLPI mRNA expression by quantitative real-time RT-PCR. The results are presented as the mean SD.
    Biotinylated Anti Mouse Slpi Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+anti+mouse+slpi+ab/Mouse+SLPI+Biotinylated+Antibody/pm18322212-61-12-24
    Average 94 stars, based on 1 article reviews
    biotinylated anti mouse slpi ab - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    FIGURE 1. Expression of SLPI during mycobacterium infection. A, Wild-type mice were intratracheally infected with BCG (4 105 CFU). At the indicated periods, total RNA was extracted from the lungs. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. B and C, At 2 days after intratracheal infection with BCG, lung tissue sections were stained with an anti-SLPI Ab (red) and 4,6-diamidino-2-phenylindole (blue) and visualized by fluorescence microscopy. D, BALF was collected at the indicated periods after BCG infection. Mouse SLPI protein expression was analyzed by Western blotting with an anti-SLPI Ab. Data obtained from two independent mice (0, 1, and 2 days) are indicated. E, AEC were incubated with the same number of BCG for the indicated periods. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. F, AEC were incubated with the same number of BCG. Culture supernatants were collected before () and after 24 h of infection () and subjected to Western blot analysis using an anti-SLPI Ab. Data obtained from two independent cell clones are shown. G, Alveolar macrophages were collected from uninfected wild-type mice, cultured with or without BCG for 48 h, and then analyzed for their SLPI mRNA expression by quantitative real-time RT-PCR. The results are presented as the mean SD.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.

    doi: 10.4049/jimmunol.180.6.4032

    Figure Lengend Snippet: FIGURE 1. Expression of SLPI during mycobacterium infection. A, Wild-type mice were intratracheally infected with BCG (4 105 CFU). At the indicated periods, total RNA was extracted from the lungs. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. B and C, At 2 days after intratracheal infection with BCG, lung tissue sections were stained with an anti-SLPI Ab (red) and 4,6-diamidino-2-phenylindole (blue) and visualized by fluorescence microscopy. D, BALF was collected at the indicated periods after BCG infection. Mouse SLPI protein expression was analyzed by Western blotting with an anti-SLPI Ab. Data obtained from two independent mice (0, 1, and 2 days) are indicated. E, AEC were incubated with the same number of BCG for the indicated periods. SLPI mRNA expression was analyzed by quantitative real-time RT-PCR. Data are shown as the relative mRNA levels normalized by the corresponding 18S rRNA level. F, AEC were incubated with the same number of BCG. Culture supernatants were collected before () and after 24 h of infection () and subjected to Western blot analysis using an anti-SLPI Ab. Data obtained from two independent cell clones are shown. G, Alveolar macrophages were collected from uninfected wild-type mice, cultured with or without BCG for 48 h, and then analyzed for their SLPI mRNA expression by quantitative real-time RT-PCR. The results are presented as the mean SD.

    Article Snippet: After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems).

    Techniques: Expressing, Infection, Quantitative RT-PCR, Staining, Microscopy, Western Blot, Incubation, Clone Assay, Cell Culture

    FIGURE 3. SLPI associates with BCG. SLPI and BSA were labeled with FLUOS (Roche). Labeled proteins were incubated with BCG for 30 min, and analyzed by fluorescence microscopy.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.

    doi: 10.4049/jimmunol.180.6.4032

    Figure Lengend Snippet: FIGURE 3. SLPI associates with BCG. SLPI and BSA were labeled with FLUOS (Roche). Labeled proteins were incubated with BCG for 30 min, and analyzed by fluorescence microscopy.

    Article Snippet: After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems).

    Techniques: Labeling, Incubation, Microscopy

    FIGURE 4. SLPI disrupts the BCG cell membrane. A, BCG was incubated with or without SLPI for the indicated periods and observed with scanning electron microscopy. B, The indicated concentrations of SLPI were added to a BCG suspension containing NPN, and the NPN fluorescence was moni- tored for the indicated periods. Repre- sentative data of three independent ex- periments are shown.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.

    doi: 10.4049/jimmunol.180.6.4032

    Figure Lengend Snippet: FIGURE 4. SLPI disrupts the BCG cell membrane. A, BCG was incubated with or without SLPI for the indicated periods and observed with scanning electron microscopy. B, The indicated concentrations of SLPI were added to a BCG suspension containing NPN, and the NPN fluorescence was moni- tored for the indicated periods. Repre- sentative data of three independent ex- periments are shown.

    Article Snippet: After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems).

    Techniques: Membrane, Incubation, Electron Microscopy, Suspension

    FIGURE 7. Slpi/ mice are highly susceptible to M. tuberculosis infection. A, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/ mice and their survival was monitored. B, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Potent antimycobacterial activity of mouse secretory leukocyte protease inhibitor.

    doi: 10.4049/jimmunol.180.6.4032

    Figure Lengend Snippet: FIGURE 7. Slpi/ mice are highly susceptible to M. tuberculosis infection. A, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/ mice and their survival was monitored. B, M. tuberculosis (4 105 CFU) were intratracheally infected into wild-type and Slpi/

    Article Snippet: After blocking with 5% milk, the membrane was incubated with the above-described biotinylated anti-mouse SLPI Ab (0.2 g/ml) and a streptavidin-HRP complex (1/10,000 dilution; R&D Systems).

    Techniques: Infection